A2831
Sigma
Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse
clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution
| Synonym: | Anti-Dinitrophenyl, Monoclonal Anti-DNP |
Description
| Analysis Note | Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin. |
| Immunogen | DNP-KLH conjugate |
| Physical form | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide |
| Application | The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE. |
Properties
| antibody form | purified immunoglobulin |
| clone | SPE-7 (monoclonal) |
| form | buffered aqueous glycerol solution |
| application(s) | direct ELISA: 1:25,000 using DNP-BSA |
| dot blot: 1:180,000 using 50 ng DNP-BSA/dot (BCIP/NBT) or 20 ng DNP-BSA/dot (chemiluminescence) | |
| isotype | IgE |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 1 |
References
| reference | Eshhar, Z., et al., Generation of hybridomas secreting murine reaginic antibodies of anti-DNP specificity. J. Immunol. 124, 775, (1980) |
